寶子們！科研大揭秘來啦！上海交通大學(xué)醫(yī)學(xué)院附屬瑞金醫(yī)院的研究發(fā)現(xiàn)，維生素 C 可不只是普通的營(yíng)養(yǎng)素，它能直接修飾蛋白質(zhì)賴氨酸殘基，特別是讓信號(hào)轉(zhuǎn)導(dǎo)和轉(zhuǎn)錄激活因子 1（STAT1） 的賴氨酸 298 位點(diǎn)發(fā)生 vitcylation，增強(qiáng)干擾素信號(hào)轉(zhuǎn)導(dǎo)，從而激活咱們身體的抗腫瘤免疫反應(yīng)，這對(duì)提升免疫力簡(jiǎn)直太關(guān)鍵啦！

維生素C

維生素C（也稱為抗壞血酸）是一種水溶性維生素，具有抗氧化特性，在人體多種生理過程中發(fā)揮關(guān)鍵作用，如參與膠原蛋白合成、促進(jìn)鐵吸收以及增強(qiáng)免疫功能等。

其實(shí)，維生素家族里的其他成員也都各有神通，在守護(hù)我們健康這件事上，一個(gè)都沒落下?；谶@一前沿成果，北京索萊寶攜旗下豐富的維生素家族產(chǎn)品，邀您一同擁抱健康新高度。

實(shí)驗(yàn)案例一：

I  F 33.8990

文獻(xiàn)

Sun L, Zhang Y, Chen G, Ji Y, Ma Q, Qiao X, Wu S, Zhou L, Bu J, Zhu X, Zhang X, Jiang X, Liu C, Li X, Liu Y, Yang Y, Liu C.

Targeting SOST using a small-molecule compound retards breast cancer bone metastasis.

Mol Cancer. 2022 Dec 29;21(1):228.

DOI:10.1186/s12943-022-01697-4.

PMID:36581888.

PMCID: PMC9798707.

方法 誘導(dǎo)小鼠MC3T3-E1 cells成骨分化；50 μg/mL L-ascorbic acid

Sun L, et al. doi: 10.1186/s12943-022-01697-4.

實(shí)驗(yàn)案例二：

I  F 36.3009

文獻(xiàn)

Ren J, Zhang Z, Geng S, Cheng Y, Han H, Fan Z, Dai W, Zhang H, Wang X, Zhang Q, He B.

Molecular Mechanisms of Intracellular Delivery of Nanoparticles Monitored by an Enzyme-Induced Proximity Labeling.

Nanomicro Lett. 2024 Feb 1;16(1):103.

DOI:10.1007/s40820-023-01313-0.

PMID:38300384.

PMCID: PMC10834923.

方法

Mouse macrophage cell line J774A.1 ：

制備了與辣根過氧化物酶（HRP）偶聯(lián)的聚乳酸 - 羥基乙酸共聚物納米顆粒作為模型（HRP (+)-PNPs），以評(píng)估納米藥物在巨噬細(xì)胞中的遞送分子機(jī)制。
The reaction was quenched after 1 min by the addition of a 100 X "quencher solution," comprising 1 M sodium azide, 1 M sodium ascorbate, and 500 mM Trolox in PBS.

Ren J, et al. doi: 10.1007/s40820-023-01313-0.

實(shí)驗(yàn)案例三：

I  F 25.9002

文獻(xiàn)

Wang P, Zhang L, Chen S, Li R, Liu P, Li X, Luo H, Huo Y, Zhang Z, Cai Y, Liu X, Huang J, Zhou G, Sun Z, Ding S, Shi J, Zhou Z, Yuan R, Liu L, Wu S, Wang G.

ANT2 functions as a translocon for mitochondrial cross-membrane translocation of RNAs.

Cell Res. 2024 Jul;34(7):504-521.

DOI:10.1038/s41422-024-00978-5.

Epub 2024 May 29.

PMID:38811766.

PMCID:PMC11217343.

方法

合成蛋白質(zhì)的疊氮高丙氨酸（AHA）標(biāo)記：

5mM sodium ascorbate (Solarbio) for 2 h

Experimental procedures for AHA labeling of synthesized proteins were adapted from a published protocol. The sample was incubated at 95 °C for 10 min. Click reaction was then performed after the samples were cooled to 37 °C in a reaction system containing 50 μMAcety-lene-PEG4-Biotin, 0.1 mM GuSO4,0.5 mM THPTA , and

5mM sodium ascorbate (Solarbio) for 2 h. The reaction was terminated with 1 mM EDTA. The labeled proteins were resolved by SDS–PAGE and detected with Biotin detection reagents.

Wang P, et al. doi: 10.1038/s41422-024-00978-5.

Wang P, et al. doi: 10.1038/s41422-024-00978-5.

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